Electroporated retinae were harvested at P31 after electroporation and dissected under a fluorescent microscope (Leica, spm8) to select EGFP+ retinae. Dissected retinae were fixed with 4% paraformaldehyde in PBS for 2 hours at 4°C, cryoprotected in PBS containing 30% sucrose for several hours at 4°C, and embedded in optimum cutting temperature compound (Sakura, Torrance, CA, USA) on dry ice. Cryosections (18 μm) were cut on a cryostat (Leica, CM3050S). Sections were stained with anti-swOPN (1:500; Millipore, catalog no. AB5405), anti-mwOPN (1:500; Millipore, catalog no. AB5407), and anti-GNAT1 antibodies (1:200; Santa Cruz Biotechnology, sc-389). Sections were incubated with secondary antibody Alexa Fluor 568 goat anti-rabbit IgG (H+L) (1:500; Thermo Fisher, catalog no. A11036) for fluorescence detection.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.