Electroporated retinae were harvested at P31 after electroporation and dissected under a fluorescent microscope (Leica, spm8) to select EGFP+ retinae. Dissected retinae were fixed with 4% paraformaldehyde in PBS for 2 hours at 4°C, cryoprotected in PBS containing 30% sucrose for several hours at 4°C, and embedded in optimum cutting temperature compound (Sakura, Torrance, CA, USA) on dry ice. Cryosections (18 μm) were cut on a cryostat (Leica, CM3050S). Sections were stained with anti-swOPN (1:500; Millipore, catalog no. AB5405), anti-mwOPN (1:500; Millipore, catalog no. AB5407), and anti-GNAT1 antibodies (1:200; Santa Cruz Biotechnology, sc-389). Sections were incubated with secondary antibody Alexa Fluor 568 goat anti-rabbit IgG (H+L) (1:500; Thermo Fisher, catalog no. A11036) for fluorescence detection.

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