Larvae from bucket crosses were collected and pooled in blue water [density of > 5 ml per larva (pH 7.2); sodium bicarbonate buffer, methylene blue (1 g/liter), and instant ocean salt (0.2 g/liter)]. Live embryos were selected ~24 hours post-fertilization (hpf) and transferred to the automated growth system in 2-liter tanks (fig. S1). The automated growth system exchanged 50% of the larvae’s growth water [> 5 ml per larva (pH 7.2); sodium bicarbonate buffer and instant ocean salt (0.2 g/liter), continuously oxygenated] four times daily and fed the larvae starting at 4 dpf with a bolus of > 30 ml of fresh paramecium culture [optical density at 490 nm (OD490) ≥ 0.2] 10 min after each water exchange. The automated system was maintained at 28°C and at a 14-hour light/10-hour dark cycle.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.