Single-cell suspension were washed and resuspended in sorting medium [DMEM; 1:100 penicillin and streptomicin (17-602F, Lonza), 1:100 l-glutamine (17-605E, Lonza), Hepes, 2 mM EDTA, DNase1 (800 U/ml), and 10 uM Y27632 (Selleck Chemicals)]. Single GFP+ cells were FACS-sorted using either FACSMelody or FACSJazz cell sorters (BD Biosciences). Living cells were discriminated by propidium iodide exclusion staining.

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