The cells were fixed with 4% PFA for 10 min and permeabilized by 0.2% Triton X-100 for 5 min at room temperature. F-actin was stained with 100 nM FITC-phalloidin for 30 min in the dark. Actin fragments were imaged with a 100× oil immersion objective on a Nikon A1Rsi super-resolution laser scanning confocal microscope (Nikon).

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