Trial experiments or similar experiments done previously were used to assess sample size with adequate statistical power. Statistical significance in SA-β-gal staining, PDLs, and BrdU incorporation assay was assessed by two-tailed Student’s t test. The correlation of PES1 expression with telomerase activity and telomere length was determined using the Mann-Whitney test. The correlation between PES1 expression and SA-β-gal staining in clinical samples was determined using Fisher’s exact test. All statistical tests were two-sided. Statistical calculations were performed using SPSS 18.0. In all assays, P < 0.05 was considered statistically significant.

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