Total RNA was extracted using TRIzol reagent according to the manufacturer’s instructions (Invitrogen). First-strand cDNA was reverse-transcribed with random primers using Moloney murine leukemia virus reverse transcriptase (Promega). The first-strand cDNA was used for PCR amplification with the following primers: hTR forward, 5′-GCCTGCCGCCTTCCACCGTTCATT-3′; hTR reverse, 5′-GACTCGCTCCGTTCCTCTTCCTG-3′; human β-actin forward, 5′-AGAAGAGCTACGAGCTGCCTGA-3′; human β-actin reverse, 5′-CAATGATCTTGATCTTCATTGTGCT-3′; mouse TR forward, 5′-ACCTAACCCTGATTTTCATTAGC-3′; mouse TR reverse, 5′-GCTCTTCGCGGCGGCAGC-3′; mouse β-actin forward, 5′-ATGGATGACGATATCGCTGCG-3′; mouse β-actin reverse, 5′-CCTTCTGACCCATTCCCACC-3′; interleukin-6 (IL-6) forward, 5′-AGGAGAAGATTCCAAAGATGTAGCCGCCC-3′; IL-6 reverse, 5′-TCTGCCAGTGCCTCTTTGCTGCT-3′; MCP1 forward, 5′-CAGCATGAAAGTCTCTGCCGCCCT-3′; MCP1 reverse, 5′-TAGCTCGCGAGCCTCTGCACT-3′; interferon-β (IFN-β) forward, 5′-AATTGAATGGGAGGCTTGAATACTGCCTCAAGG-3′; IFN-β reverse, 5′-GTCTCATTCCAGCCAGTGCTAGATGAATCTTGT-3′; IκBα forward, 5′-TCGGAGCCCTGGAAGCAGCA-3′; IκBα reverse, 5′-AGTCTGCTGCAGGTTGTTCTGGAAGTTG-3′; human pre-tRNA-Leu forward, 5′-GTCAGGATGGCCGAGTGGTCTAAG-3′; human pre-tRNA-Leu reverse, 5′-CCACGCCTCCATACGGAGAACCAGAAGACCC-3′; human pre-tRNA-Arg forward, 5′-GGCTCTGTGGCGCAATGGATA-3′; human pre-tRNA-Arg reverse, 5′-TTCGAACCCACAACCTTTGAATTGCTC-3′; human pre-tRNA-Tyr forward, 5′-CCTTCGATAGCTCAGCTGGTAGAGCGGAGG-3′; human pre-tRNA-Tyr reverse, 5′-CGGAATTGAACCAGCGACCTAAGGATGTCC-3′. β-Actin was used as an internal control. The relative expression was calculated by the comparative Ct method.

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