MCF7 cells at G1 phase were obtained by treating cells with aphidicolin (10 μg/ml) for 14 hours. After extensive washing by phosphate-buffered saline (PBS), cells were released from G1 phase by adding fresh medium, and S phase cells were collected 3 hours later. For G2-M phase cells, cells were treated with nocodazole (50 ng/ml) for 14 hours and subjected to mechanical shake-off. Detached cells were M phase cells while rest cells were G2 cells. The cell phases were examined by flow cytometry.

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