Immunofluorescence was performed as previously described (15). Briefly, cells grown on glass coverslips were fixed, permeabilized, and blocked in normal goat serum. The coverslips were then incubated with primary antibodies, followed by incubation with corresponding secondary antibodies. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Confocal images were collected using an LSM 780 confocal microscope (Zeiss).

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