Comet assay was performed as previously described (49). Briefly, cells were treated as indicated and then mixed gently with premelted low-temperature–melting agarose at a volume ratio of 1:1 (v/v) and spread on glass slides. The slides were then submerged in precooled lysis buffer at 4°C for 90 min. After rinsing, the slides were electrophoresed in running buffer [1 mM Na2EDTA and 300 mM NaOH (pH 13.0)] at 1.0 V/cm for 20 min and then stained with propidium iodide (5 μg/ml). Fluorescent images of more than 100 nuclei were captured under an Olympus FV1000-IX81 confocal microscope (Tokyo, Japan).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.