The human SIRT7 expression constructs (WT SIRT7 and H187Y SIRT7) were gifts from K. F. Chua (Stanford University, USA). The following siRNAs were used to silence target genes by transfecting with Lipofectamine 2000 (Life Technologies–Invitrogen, USA), according to the manufacturer’s instructions:

scrambled siRNA, UUCUCCGAACGUGUCACGU; SIRT7 siRNA1, AGCCATTTGTCCTTGAGGAA; SIRT7 siRNA2, GAACGGAACTCGGGTTATT.

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