This protocol is extracted from research article:
Intra- versus intergroup variance in collective behavior
Sci Adv, Jan 2, 2019; DOI: 10.1126/sciadv.aav0695

Desert locusts, Schistocerca gregaria (Forskål), were obtained from our colony at the School of Zoology, Tel Aviv University, Israel. The locusts were reared for many consecutive generations under crowded conditions with 100 to 160 individuals in 60-liter aluminum cages under a controlled temperature of 30°C, 35 to 60% humidity, and a 12-hour dark/12-hour light cycle. The locusts were fed daily with wheat seedlings and dry oats. All experiments were performed with nymphs of the final (fifth) nymphal instar (3 to 4 cm in length and ~0.5 cm in width).

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