Robo2-shRNA–encoding lentivirus was prepared as previously described (11), using packaging and envelope plasmids (Addgene plasmid #12260 and #12259) and a lentivirus vector encoding Robo2 shRNA or a Cnt shRNA with a nontarget sequence (Open Biosystems, Huntsville, AL, USA). Each animal was given stereotaxic injections of 1.8 μl of lentivirus into the medial striatum of the right hemisphere (relative to bregma: anterior, 0.9 mm; lateral, 1.25 mm; depth, 2.6 to 2.0 mm) 2 days before stroke induction and then was fixed 20 days later.

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