Whole blood samples were diluted 10 times with sterile 1 × phosphate-buffered saline. Then, the diluted whole blood samples were kept bubbling with oxygen gas or air for 2 hours at 4°C. Then, 1.5 μl of the whole blood was pipetted onto a poly-l-lysine–coated cover slide and sandwiched between a cover slide (0.17-mm thickness; VWR Micro Cover Glasses, 48393-048) and poly-l-lysine–coated cover slide (P0425-72 EA, Sigma-Aldrich). We waited for at least 30 min so that the RBC could adhere to the surface of the poly-l-lysine–coated cover slide because of the electrostatic force.

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