For comparison of multiple groups, one-way analysis of variance (ANOVA) with Tukey’s multiple comparison test and Dunnett’s multiple comparison test were assessed using Prism software. P values less than 0.05 were considered significant. The significance level is displayed as asterisks (*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001; n.s. not significant). For all comparisons, samples were not excluded from data except only for tonic GABA current recording; cells with holding currents over −100 pA were excluded, and experiments were stopped after 3 hours from the time of slicing. No animal was excluded from analysis in behavioral experiments. When using either sex, different litters, or ages of animals, animals were evenly allocated to each experimental group, and for slice immunostaining and electrophysiological experiments, we randomly conducted samples in each experimental group. Detailed results for ANOVA are described in table S6.

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