The indicated cells were stained with 50 nM LysoTracker Red (Thermo Fisher Scientific, L7528) in DMEM for 30 min at 37°C and washed with PBS three times. Cells were then resuspended in fluorescence-activated cell sorting (FACS) buffer [1% FBS, 1 mM EDTA, and 25 mM Hepes (pH 7) in PBS] containing 4′,6-diamidino-2-phenylindole (DAPI) (10 μM/ml) (Thermo Fisher Scientific) before analysis by flow cytometry (FACSymphony, BD Biosciences).

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