All mice were kept in a temperature- and humidity-controlled environment with a 12-hour light/12-hour dark cycle, and mice had free access to food and water. Handing and animal care was performed according to the directives of the Animal Care and Use Committee of the Institutional Animal Care and Use Committee of Korea Institute of Science and Technology (KIST) (Seoul, Korea). APP/PS1 mice of B6C3 hybrid background originated from the Jackson Laboratory (USA; stock number 004462) and were maintained as hemizygotes by crossing transgenic mice to B6C3 F1 mice, which were derived from a cross between a C57BL/6 female and a C3H male. This hybrid strain is commonly used in the production of transgenic mice. Both sexes of 8- to 13-month-old transgenic mice and WT littermates were used. In most behavioral experiments, mice were treated by oral administration with each concentration for selegiline or KDS2010 at 1 and 10 mg/kg per day. The amount of each drug (in mg) was calculated according to each mouse’s weight (in kg) and was dissolved in 100 μl of water. Each oral administration was performed once per day. In the case of the Morris water maze experiment, 10 mg of KDS2010 was dissolved in 50 ml of water. The mice were provided with water (control) and KDS2010 solution ad libitum. Behavioral experiments were performed during the light phase of the light-dark cycle at the same time of each day. No statistical method was used to predetermine sample size. When using either sex, different litters, or ages of animals, animals were evenly allocated to each experimental group. All experiments were done with gender- and age-matched controls. Behavioral tests were conducted in a blind manner. The investigator was blinded to the group allocation of the genotype or treatment during the experiment and when assessing all testing.

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