NDR2f/f mice, a C57BL/6J background, were gifts from B. Hemmings (Friedrich Miescher Institute for Biomedical Research). Lysm-Cre mice C57BL/6J were provided by L. Wang (Zhejiang University School of Medicine). Mice genotype identification was performed by polymerase chain reaction (PCR) analysis of DNA isolated from the tail using the following primers: NDR2-F, 5′-CCTCAGACTTTTTGTGATCCTCC-3′; NDR2-R, 5′-AGCTCAACCTCCCAACTCAG-3′; Lysm-mut, 5′-CCAGAAATGCCAGATTACG-3′; Lysm-com, 5′-CTTGGGCTGCCAGAATTTCTC-3′; and Lysm-wt, 5′-TTACAGTCGGCCAGGCTGAC-3′. All mice were maintained under specific pathogen–free conditions in the University Laboratory Animal Center. All animal experiments were approved by the Ethical Review Committee of Zhejiang University School of Medicine and were in compliance with institutional guidelines.

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