PBS-buffered, formalin-fixed, paraffin-embedded samples were sectioned (5 μm thick) for H&E staining and immunohistochemistry (IHC). To gain better histology of testis seminiferous tubules, testes were fixed in Bouin’s solution and subjected to H&E staining. For IHC, sections were deparaffinized and rehydrated. Primary antibodies were applied at suitable dilutions (table S3) at room temperature for 1 hour and then incubated with biotinylated secondary antibodies for 30 min. Sections were then stained using Vectastain ABC (anti-biotin complex) and DAB (3, 3-diaminobenzidine) peroxidase substrate kits (Vector Laboratories, Burlingame, CA, USA). Antibodies used in this study are listed in table S3.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.