Mouse myoblast cell line (C2C12) was acquired from the American Type Culture Collection. The cells were maintained at subconfluent densities in GM at 37°C in a tissue culture incubator with a constant supply of 5% CO2. GM consists of Dulbecco’s modified Eagle medium (DMEM; Gibco) supplemented with 10% FBS and 1× antibiotic-antimycotic (Life Technologies). For myogenic differentiation assay, the myoblast cells were grown to about 70% confluency, washed with phosphate-buffered saline (PBS), and cultured with DM. DM consists of DMEM containing 2% heat-inactivated horse serum (HyClone) and 1× antibiotic-antimycotic. Cells were harvested while growing in GM and after 24 and 96 hours (DM1 and DM4, respectively) in DM.

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