For RT-qPCR, we compared data for pairs of groups in Mann-Whitney tests (expression in fibroblasts from patients versus that in control fibroblasts, with n = 5 each) and unpaired t tests (overexpression and siRNA assays with n > 20 per condition). We considered P values below 0.05 to indicate statistical significance. The analyses were performed with GraphPad Prism version 6.00 (GraphPad Software, La Jolla, CA, USA).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.