Two SNPs from the MEG3 and H19 gDNA and cDNA were sequenced by standard Sanger sequencing methods, with the ABI PRISM BigDye Terminator v3.0 Cycle Sequencing Kit and the ABI 3100 Genetic Analyzer. The sequencing products were then analyzed with SeqScape v2.6 (Life Technologies, Courtaboeuf, France).

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