DNA was extracted from blood leukocytes and from skin-derived fibroblast cultures, by an in-house protocol, after cell lysis by a salting-out procedure, as previously described (28, 29). Total RNA and miRNA were extracted from cultured fibroblasts with the NucleoSpin miRNA Kit for the isolation of small and large RNAs (MACHEREY-NAGEL, France). Both DNA and RNA were quantified with a NanoDrop ND-1000 spectrophotometer (Invitrogen, France).

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