Skin-derived fibroblasts from patients and controls were cultured to confluence in RPMI 1640 (Gibco, Cergy Pontoise, France) supplemented with 10% fetal calf serum and ampicillin (50 U/ml)/streptomycin (50 μg/ml) at 37°C (number of passages, <10). Cells were then treated with trypsin and centrifuged. The cell pellet obtained was washed twice with 1× phosphate-buffered saline and used for DNA and micro/large-scale RNA extractions.

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