Skin-derived fibroblasts from patients and controls were cultured to confluence in RPMI 1640 (Gibco, Cergy Pontoise, France) supplemented with 10% fetal calf serum and ampicillin (50 U/ml)/streptomycin (50 μg/ml) at 37°C (number of passages, <10). Cells were then treated with trypsin and centrifuged. The cell pellet obtained was washed twice with 1× phosphate-buffered saline and used for DNA and micro/large-scale RNA extractions.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.