Nalm6, Jurkat, and 658w WT and casp-3/7−/− cells were plated in 3 ml of RPMI 1640 complete medium in six-well plates at a concentration of 2 × 105 cells per well. The cells were then treated for 24 to 48 hours with either Birinapant (50 nM) and TNF (10 ng/ml), ABT263 (5 mM), or vehicle. The cells were then collected, washed with PBS, mixed with Methocult Optimum media (catalog no. 4034; StemCell Technologies), and plated at a concentration of 1000 cells per well in six-well SmartDish plates (catalog no. 27370; StemCell Technologies). Cells were aliquoted in duplicate samples. After 10 to 14 days, colony-forming units were visually counted under a microscope.

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