Flies were raised under a 12-hour light/12-hour dark (LD) regimen at 25°C on standard corn and yeast media. CantonS flies from R. Stanewsky (University of Münster, Germany) were used as wild-type flies, and ClkJr.k flies (Bloomington Drosophila Stock Center #24515) (57) were used as a clock mutant. To estimate the activity on the R2 neurons of the ellipsoid body, we used the R30G03-GAL4 (Bloomington Drosophila Stock Center #49646) (51) strain in combination with CaLexA (50). All analyzed animals were socially naïve, unless otherwise stated.

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