Flies were raised under a 12-hour light/12-hour dark (LD) regimen at 25°C on standard corn and yeast media. CantonS flies from R. Stanewsky (University of Münster, Germany) were used as wild-type flies, and ClkJr.k flies (Bloomington Drosophila Stock Center #24515) (57) were used as a clock mutant. To estimate the activity on the R2 neurons of the ellipsoid body, we used the R30G03-GAL4 (Bloomington Drosophila Stock Center #49646) (51) strain in combination with CaLexA (50). All analyzed animals were socially naïve, unless otherwise stated.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.