The method for calculation of angle displacement at molecular level is illustrated in fig. S8. First, each starting structure was preprocessed to filter out noise and obtain a cleaner structure for data extraction. A three-way moving average filter was used. Overall, structure patterns were maintained after filtering. Following filtering, 3 of 15 strands were selected in the model. These triplets were selected to best represent the overall structure, so perimeter strands were primarily used. Amino acids located at the same position in each of the three strands were selected to form a triangle. Alpha carbon positions from the amino acids on each triplet were projected onto the xy plane. At a position downstream on the strand (10 residues down in the example, see fig. S8), another triangle was formed to calculate angle displacement (and corresponding twist) between the two triangles sampled. The coordinates of the three points in both triangles were adjusted relative to the aligned centroids of the triangles. Twist angle was measured as an average of the angle displacement for the three points.

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