The study included seven European countries, where UWTP samples were collected over three sampling campaigns in early autumn 2015 and 2016 and early spring 2016. Volumes between 1 and 2 liters were collected, in sterile flasks, and stored at 4ºC until analysis for a maximum of 12 hours. In each sampling campaign, were collected three 24-hour composite samples, one per day, over three consecutive days (Tuesday, Wednesday, and Thursday) (table S1). Geographic, demographic, and technical characterization provided by the UWTPs is available in table S4. All participants followed a common protocol for sampling, sample processing, and DNA extraction.

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