Quantification and statistical analysis
This protocol is extracted from research article:
A central control circuit for encoding perceived food value
Sci Adv, Nov 21, 2018; DOI: 10.1126/sciadv.aau9180

Data were analyzed using GraphPad Prism 5 (GraphPad Software) and expressed as means ± SEM. Each “n” represents an individual preparation, unless stated otherwise in the text. Normality was tested using the D’Agostino and Pearson omnibus normality test. Where data were shown to be normally distributed, two-group statistical comparisons were performed using two-tailed t test statistics (either paired or unpaired as stated in the text). Data with more than two groups were first analyzed using a one-way analysis of variance (ANOVA), followed by a Tukey’s multiple comparison test. Non-normally distributed data were analyzed using a Mann-Whitney U test or Wilcoxon signed-rank test. The comparisons between the percentage of bites/cycles classified as ingestion or egestion were compared using a Fisher’s exact test. The significance level was set at P < 0.05.

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