D2 receptor blocker application in vitro and in vivo
This protocol is extracted from research article:
A central control circuit for encoding perceived food value
Sci Adv, Nov 21, 2018; DOI: 10.1126/sciadv.aau9180

Sulpiride (±) is an effective dopamine antagonist in Lymnaea, blocking the effects of dopaminergic neurons and focal application of dopamine (55). To test for the effect of sulpiride (±) (Sigma-Aldrich) on the PRN→B11 connection, preparations were bathed in high divalent (HiDi) saline, which increases the threshold for action potentials, acting to reduce polysynaptic connections. HiDi saline was composed of 35.0 mM NaCl, 2 mM KCl, 8.0 mM MgCl2, 14.0 mM CaCl2, and 10 mM Hepes buffer in water. Baseline EPSP size was acquired, then 10−4 M sulpiride (±) in HiDi saline was perfused into the bath for 10 min, and EPSP size was recorded again. To test the behavioral effects of sulpiride, animals were injected with 100 μl of 10−3 M sulpiride (±) in normal saline. The injected concentration of the drug was diluted ~10-fold by the body fluids of the animal. Control animals were injected with 100 μl of normal saline alone. Animals were left for 2 hours before behavioral tests were carried out.

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