Iontophoretic dye filling of neurons
This protocol is extracted from research article:
A central control circuit for encoding perceived food value
Sci Adv, Nov 21, 2018; DOI: 10.1126/sciadv.aau9180

Neurons were filled with a fluorescent dye, allowing the morphology of the cell to be determined. Microelectrodes were filled with either 5-CF or Alexa Fluor 568 (Molecular Probes). Cells were filled iontophoretically using a pulse generator, which applied regular interval negative square current pulses into the neuron for >30 min. The preparation was then left overnight at 4°C. Images of the neurons were taken using a digital camera (Andor iXon EMCCD) mounted on a Leica stereomicroscope.

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