Animal maintenance
This protocol is extracted from research article:
A central control circuit for encoding perceived food value
Sci Adv, Nov 21, 2018; DOI: 10.1126/sciadv.aau9180

All experiments were performed on adult (3 to 4 months old) Lymnaea stagnalis. Animals were kept in groups in large holding tanks containing Cu2+-free water at 20°C on a 12-hour light/12-hour dark regime. The animals were fed lettuce three times a week and a vegetable-based fish food (Tetra Phyll; Tetra Werke, Melle, Germany) twice a week. Animals were transferred to smaller holding tanks before experimenting. Animals were either fed ad libitum or food deprived for 1, 2, 3, or 4 days before either behavioral or electrophysiological experiments.

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