Human RCC cell lines (Caki-2, 786-O, A498, A704, 769-P, ACHN, RCC4+vec, and RCC4+VHL) were maintained in RPMI-1640 medium (Sigma-Aldrich) supplemented with 10% FBS, penicillin (24 U/ml), and streptomycin (25 μg/ml). Caki-2 (2.0 × 104 cells per well), 786-O (1.0 × 104 cells per well), A498 (1.0 × 104 cells per well), A704 (2.0 × 104 cells per well), 769-P (1.0 × 104 cells per well), ACHN (1.0 × 104 cells per well), RCC4+vec (0.7 × 104 cells per well), and RCC4+VHL (0.8 × 104 cells per well) cells were plated into 96-well plates 1 day before GO289 treatments. Two days after treatment, cells were stained with Hoechst 33342 (2 μg/ml; Invitrogen) for 1 hour and counted with ArrayScan XTI (Thermo Scientific).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.