PBMCs were resuspended at 1 × 106 cells/ml in FACS buffer with 20% human Fc receptor–binding inhibitor (eBioscience) and incubated for 20 min at room temperature in 5-ml polystyrene FACS tubes (BD Biosciences). The PBMCs were stained in a total volume of 135 μl per tube with 0.5 μl of anti-human CD3 (UCHT1) PE-Cy7 (eBioscience), 0.5 μl of anti-human CD4 (SK3) PerCP-eFluor 710 (eBioscience), 0.5 μl of anti-human CD8 (SK1) APC-eFluor780 (eBioscience), and anti-human CD14 (MφP9) V500 (BD Biosciences). The cells were incubated in the dark for 30 min at room temperature. They were washed twice with 3 ml of FACS buffer and resuspended in 0.5 ml of FACS buffer with 1 μM 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) (fig. S11).

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