Statistical analysis of electron miscroscopy data was analyzed using the Mann-Whitney test (independent samples and two-sided; GraphPad). Statistical analysis of immunoblotting, PCR, and fluorescence-activated cell sorting (FACS) data were performed using unpaired, two-tailed Student’s t test (Excel software). Values are shown as means ± SD of at least three independent experiments. P values <0.05 were marked by an asterisk. Densitometric analysis of immunoblots was performed using the Adobe Photoshop software. The control ratio was arbitrarily defined as 1.00. Normal distribution was assumed on the basis of the appearance of the data, since n < 5. No statistical method was used to predetermine sample size for animal studies. The animal experiments were not randomized. The investigators were not blinded to allocation during experiments and outcome assessment. No exclusion criteria were applied to exclude samples or animals from analysis.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.