We conducted an incubation experiment with three replicates using the same soils as in the field and pot experiments. As with the pot experiment, the incubation experiment consisted of three treatments: −S soils without straw, −S soils with straw, and +S soils with straw. In the straw addition treatments, fresh wheat straw (2 to 5 mm) was mixed with soils at a rate equivalent to 6 MT ha−1. We added 15 g of soil with or without straw to 40-ml beakers and kept a water layer of 2 to 3 cm. Soils were incubated at 30°C in the dark for 4 weeks.

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