The primary antibodies used in this study were rabbit anti-HA antibody (Sigma-Aldrich, H6908), rabbit anti-FLAG antibody (Sigma-Aldrich, F7425), mouse anti-MYC antibody [Santa Cruz Biotechnology, sc-40 (9E10)], rabbit anti-MYC antibody (Millipore, 06-549), rabbit anti–BECLIN 1 [Santa Cruz Biotechnology, sc-11427 (H-300)], goat anti–BECLIN 1 [Santa Cruz Biotechnology, sc-10086 (D-18)], rabbit phospho–BECLIN 1 (Ser15) [Cell Signaling Technology, 138255 and 84966 (D4B7R)], rabbit anti-ATG14 [Cell Signaling Technology, 96752 (D1A1N)], rabbit phospho-ATG14 [Cell Signaling Technology, 13155 (S29)], rabbit anti-NBR1 (Novus Biologicals, NBP1-71703), LC3 (Cell Signaling Technology, 27755), mouse anti–human AMBRA1 [Santa Cruz Biotechnology, sc-398204 (G6)], rabbit anti–human AMBRA1 (Novus Biologicals, 26190002), rabbit anti-mouse Ambra1 (Millipore, ABC131), mouse anti–multi ubiquitin (MBL International, ST1200), rabbit anti–K63-linked ubiquitin (Millipore, 05-1308), rabbit anti–K48-linked ubiquitin (Millipore, 05-1307), rabbit anti-ULK1 [Santa Cruz Biotechnology, sc-33183 (H240)], rabbit anti-ULK1 [Cell Signaling Technology, 80545 (D8H5)], rabbit anti-TRIM32 (Thermo Fisher Scientific, PA5-22316), rabbit anti–phospho-VPS34 (Ser249) (Cell Signaling Technology, 138575), rabbit anti-VPS34 (Life Technologies, 382100), mouse anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (Millipore, CB1001), and mouse monoclonal myosin heavy chain antibody MF20 (Novus Biologicals, MAB4470).

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