Myoblast preparation was obtained by transducing human fibroblasts from healthy or Trim32 mutant patients with the lentivirus cytomegalovirus (CMV) MyoD-ER(T) (Addgene) with a multiplicity of infection of 20 and treated with 1 μM 4OH-tamoxifen (Sigma-Aldrich, H7904) to activate MyoD-ER(T). The transdifferentiation was confirmed by PCR analysis of MyoD and myogenin expression. Autophagy analysis was performed in the absence of 4OH-tamoxifen.

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