All animal experiments were performed in accordance with the guidelines for the welfare of experimental animals issued by the State Government of Lower Saxony, Germany, in compliance with European and National Institutes of Health guidelines (33.9-42502-04-13/1359 and 33.9-42502-04-13/1052). Hippocampi were isolated from newborn (P0) qv3J mice, collected in a serum-free Neurobasal-A medium (Life Technologies, 12349-015) with 100 mM Hepes buffer solution (Life Technologies, 15630-056), and digested with trypsin/EDTA (0.05%/0.02%, w/v; Biochrom) in phosphate-buffered saline (PBS) for 12 min at 37°C. The hippocampi were then transferred to Neurobasal-A medium, pipetted up and down for homogenization, and centrifuged for 2 min. Hippocampi from each newborn offspring were used to prepare a separate culture. For the dataset in fig. S3E, hippocampi from embryonic day 18 (E18) wild-type mice were pooled. For electrophysiology measurements and wide-field imaging, cells from the hippocampi of each newborn mouse were plated in one 8.8-cm2 cell culture dish (Nunclon, 153066), on seven glass coverslips (Thermo Fisher Scientific, Menzel Gläser 10 mm #1), coated with poly-l-lysine (0.1 mg/ml; Sigma-Aldrich, P2636), in 2 ml of Neurobasal-A medium supplemented with 1:50 B27 (Life Technologies, 17504-044), 1:400 GlutaMAX (Life Technologies, 35050-038), and fibroblast growth factor (0.01 μg/ml; Life Technologies, 13256-029). For dSTORM imaging, the cells were seeded in four-well tissue culture chambers on cover glass (Sarstedt 94.61990.402), one hippocampus per well, in supplemented medium. Cultures were kept in an incubator at 37°C in a humidified atmosphere of 95% air and 5% CO2. One-half of the culture medium was changed weekly with freshly prepared medium. Tail biopsies were used for genotyping and were afterward related with the corresponding culture.

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