Two copies of the Fab from the Fab311-(NPNA)3 crystal structure were docked onto the cryo-EM model of rsCSP-Fab311 using UCSF Chimera. The heavy-chain hinge region was extended manually using UCSF Chimera such that the Fc plus partial hinge region crystal structure 5v4e could be docked onto it. The torsion angles in the hinge region were adjusted manually in COOT such that all of the disulfides were appropriately satisfied. The resulting IgG model was idealized and relaxed using Rosetta (45). Five copies of the IgG model were docked onto the cryo-EM model of rsCSP-Fab311 using UCSF Chimera to generate the model shown in Fig. 5C.

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