The rsCSP-Fab311 and rsCSP-Fab317 complexes, each at a concentration of 5 mg/ml, were frozen with n-dodecyl-β-d-maltopyranoside to aid in sample dispersal. Each complex and detergent were briefly incubated together and then deposited on a Solarus plasma cleaned C-flat 2/2-4C grid (Protochips) and subsequently plunge frozen using a Thermo Fisher Vitrobot Mark IV. The settings for the Vitrobot were as follows: 4°C, 100% humidity, 10-s wait time, 4.5-s blot time, and a blot force of 0.

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