The HR2 peptide samples used for NMR spectroscopy were prepared by dissolving an appropriate amount of peptide in 0.5 ml of solution (H2O/D2O = 9:1) to obtain a peptide concentration of 1.0 mM. The peptide-Y11 complex was prepared by the same procedure by mixing the two components at a ligand/peptide ratio of 1:1. The NMR spectra were recorded on a Bruker DRX-600 spectrometer equipped with a cryoprobe. 1D NMR spectra were recorded in Fourier mode with quadrupole detection. The TOCSY and NOESY (nuclear Overhauser effect spectroscopy) experiments were performed in a phase-sensitive mode using quadrupole detection in ω1 with a time-dependent phase increase in the initial pulse. The data block sizes were 2048 addresses in t2 and 512 equidistant t1 values. Before the Fourier transformation, the time domain data matrices were multiplied by shifting the sine functions in both dimensions. The TOCSY experiments were performed at 298 K with a mixing time of 200 ms. The NOESY experiments were performed at 298 K with a mixing time of 300 ms. The NMR spectra were analyzed using MestReNova software.

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