Mice were housed in temperature- and humidity-controlled facilities on 12-hour dark-light cycles with free access to food and water. The animal care and use committees of the Cincinnati Children’s Hospital Medical Center approved all animal procedures. Institutional Animal Care and Use Committee guidelines were followed with animal subjects. To study the specific function of SCs, we used Dhh-Cre transgenic mouse, where Cre-mediated recombination activity would result in deletion of the floxed Nf1 allele in SC/SCPs of the developing peripheral nerves at E12.5. We bred the Runx1fl/fl;Runx3fl/fl mice (38, 39) onto the Nf1fl/fl background to obtain F1 generation (Runx1fl/+;Runx3fl/+;Nf1fl/+). We also bred the Runx1fl/fl;Runx3fl/fl mice with Nf1fl/+;DhhCre+ mice to obtain Runx1fl/+;Runx3fl/+;Nf1fl/+;DhhCre mice. We interbred Runx1fl/+;Runx3fl/+;Nf1fl/+ mice to obtain Runx1fl/fl;Runx3fl/fl;Nf1fl/fl mice. We then bred Runx1fl/fl;Runx3fl/fl;Nf1fl/fl mice with Runx1fl/+;Runx3fl/+;Nf1fl/+;DhhCre mice to obtain Runx1fl/fl;Runx3fl/fl;Nf1fl/fl;DhhCre mice. Littermates Runx1fl/+;Runx3fl/+;Nf1fl/fl;DhhCre, Runx1fl/+;Runx3fl/fl;Nf1fl/fl;DhhCre, Runx1fl/fl;Runx3fl/+;Nf1fl/fl;DhhCre, or Runx1fl/fl;Runx3fl/fl;DhhCre mice were used as controls. Genotyping was performed as described (16).

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