The light transmittance of the cleared whole brain (Fig. 3C) was measured with a visible near-infrared optical fiber spectrometer (USB4000, Ocean Optics, USA). A circular spot of light (diameter, 5 mm) was irradiated on the central part of cleared brain samples from the dorsal side and was measured from the ventral side. The blank value was measured as the transmittance of the clearing reagents without a sample. The light transmittance of the sample normalized to the blank value, which was 100%, was defined as the relative transmittance. Each value was determined as an average of three measurements.

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