We labeled the vasculature in C57BL/6N mouse brain and kidney with DyLight 649 L. esculentum (Tomato) lectin (LEL-Dylight649, DL-1178, Vector Laboratories) or Alexa Fluor 647 anti-mouse CD31 antibody (CD31-A647, 102416, BioLegend) by caudal vein injection. LEL-Dylight649 was diluted in sterile saline to a concentration of 0.5 mg/ml (0.1 ml per mouse) and used to label the blood capillaries in the brain. The Alexa Fluor 647 anti-mouse CD31 antibody (10 to 15 μg) was diluted in sterile saline (total volume of 200 μl) to trace blood vessels in various diameters. After injection, the animals were placed in a warm cage for 30 min prior to perfusion. The mouse brains and kidneys were collected after perfusion.

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