THP-1 was stimulated every other day for 12 days with human interleukin-2 (100 U/ml). The HL-60 cell line was treated with 1.3% dimethyl sulfoxide for 96 hours, as previously reported to differentiate into polymorphonuclear-like cells. DiI was used to stain THP-1 or HL-60 as a membrane marker for 30 min, followed by DiI quenching before the experiment. Cy5-cRGDLs were incubated with THP-1 or HL-60 for 1 hour, and then the cRGDLs that were not taken up by the cells were completely removed. THP-1-cRGDLs or HL-6-cRGDLs (1 × 104 cell/cm2) were added into the PC12 cells (1 × 104 cell/cm2). After 1 hour of incubation, the interactions between cells were observed by CSLM.

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