The statistical significance of the data was examined by Student’s t test or Kolmogorov-Smirnov test for comparisons between two groups, or by one-way analysis of variance (ANOVA) with Bonferroni’s multiple comparison test for analyses of three or more groups using Microsoft Excel 16 or GraphPad Prism 6 unless otherwise indicated. Data are presented as means ± SEM. Results were considered statistically significant if P < 0.05.

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