For nonpermeable immunofluorescence experiments, procedures were similar to regular immunofluorescence as above, with the exception that both blocking and antibody incubations were performed in PBS with 5% goat serum only. Ten individual neurons, each from three independent preparations of cortical GABAergic interneurons, were used to derive the data presented. Between two and five distinct 10-μm segments from each neuron were randomly chosen and quantified for the number of nonpermeable immunofluorescence puncta, as detected by an antibody recognizing the extracellular domain of GABAA receptor β//2/3 subunit.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.