For nonpermeable immunofluorescence experiments, procedures were similar to regular immunofluorescence as above, with the exception that both blocking and antibody incubations were performed in PBS with 5% goat serum only. Ten individual neurons, each from three independent preparations of cortical GABAergic interneurons, were used to derive the data presented. Between two and five distinct 10-μm segments from each neuron were randomly chosen and quantified for the number of nonpermeable immunofluorescence puncta, as detected by an antibody recognizing the extracellular domain of GABAA receptor β//2/3 subunit.

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