Tissue homogenates were prepared as described above, and protein concentration was measured by BCA assay. Ten micrograms of total protein was mixed with sample buffer and a final concentration of 1% sarkosyl or digitonin for analysis of GABARAPL2 and GABARAP, respectively. Samples were separated on NativePAGE 3-12% Bis-Tris gels and processed in the same manner as SDS-PAGE.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.