AAV was produced by transfection of pAAV2-CaMKII promoter-MCS-IRES2-Venus or pAAV2-CaMKII promoter-CreHA-IRES2-Venus plasmids into AAV-293 cells with pAdΔF6 and pAAV2/1 plasmids. AAVs were purified by a discontinuous iodixanol gradient, as described previously (46). AAV titer was determined by quantitative PCR. Stereotaxic injections were bilaterally placed at 2.43 mm anterior to the bregma, 0.3 mm lateral to the sagittal suture, and 1.2 mm below the skull surface in 12-week-old CaMKII-cre Atg7flox/flox mice. One microliter of lentivirus (5.0 × 1012 genome copy/ml) was injected at a rate of 0.1 μl/min by a microinjector (Narishige).

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