Protein concentrations were determined with the BCA Protein Assay Kit (Bio-Rad Laboratories, Hercules, CA, USA and Thermo Fisher Scientific, Waltham, MA, USA), with bovine serum albumin used as the standard. SDS–polyacrylamide gel electrophoresis was carried out according to Laemmli, and gels were stained with Coomassie Brilliant Blue. All other chemicals were obtained from Sigma-Aldrich Japan KK (Tokyo, Japan) or Wako Pure Chemicals Industry Ltd. (Osaka, Japan). For crystallization screening, chemicals were obtained from Hampton Research Corp.

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